Prestained Protein Marker, Broad Range (6-175 kDa)

	Catalog #		Size		Concentration
	P7708S   STOCK!		175 mini-gel lanes
	P7708L		875 mini-gel lanes

10-20% SDS-PAGE

Description:
Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands when electrophoresed. The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below. For precise molecular weight determinations, use NEB's unstained Protein Marker, Broad Range (NEB #P7702) in addition to the prestained marker.

Usage notes:

1. Mix protein marker. Bring desired amount of the Prestained Protein marker over to a separate tube. For blotting: use 6 μl for mini-gels and 12 μl for full length gels. For visualizing during electrophoresis: use 15 μl for mini-gels and 30 μl for full length gels.
2. Heat the Marker to 95-100°C for 3-5 minutes.
3. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells.