Applications

• Quantitative RT-PCR
• RT-PCR
• Northern Blot Analysis
• Microarray Target Labeling
• In Vitro Transcription Reactions
• Ribonuclease Protection Assays
• cDNA synthesis and Library construction

Highly Pure and DNA-free Total RNA

RNA quality and purity are paramount for successful Northern, RT-PCR, microarray target labeling or QRT-PCR experiments. Traces of phenol, DNA contamination, and RNA degradation can contribute to poor labeling and signal quality in microarray experiments as well as inhibit RT-PCR or QRT-PCR amplification. Using intact, DNA- free, pure total RNA improves detection and discrimination of low expression level genes in virtually every application. For example, our StratageneŽ Universal Human Reference RNA (UHRR) is prepared using the Absolutely RNAŽ Kit method modified for large scale isolation and has undergone extensive quality control procedures to ensure batch-to-batch consistency

Fast, Easy, Non-Toxic Method

Our Absolutely RNA kits use a safe, rapid, phenol-free method that works with tissue or cultured cells. The method for all kits is simple. Isolation of total RNA is performed in a single spin-cup to minimize potential loss of RNA during the procedure. Following lysis, the solution is mixed with ethanol and applied to the silica-based fiber matrix within the spin-cup to bind the RNA. DNA contamination is effectively removed by incubation with DNase I. The RNA is then washed to remove contaminants and total RNA is recovered in the appropriate final elution buffer

Purify RNA from Laser Microdissected Cells

Laser microdissection (LMD) is a technique used to isolate a pure subpopulation of cells from tissue sections containing a heterogeneous mixture for detailed molecular analysis. Isolating high quality total RNA from LMD-recovered cells can be challenging and inefficient when standard methods are used. In contrast, our Absolutely RNAŽ Microprep Kit and Absolutely RNAŽ Nanoprep Kit easily isolate pure RNA from small samples without the need for additional reagents or precipitation. Using our specially designed spin cups for 10ul elution volume, the Absolutely RNAŽ Nanoprep Kit is the only kit qualified to isolate RNA from a single cell.

Complete Kits Deliver High Quality RNA, Convenience and Savings

All of our Absolutely RNA isolation kits have all the necessary reagents including DNase I to isolate pure total RNA, saving you money and hassle in the ordering process. Additionally, our DNase is supplied lypophilized which makes our kits perfect for room temperature storage that saves valuable freezer space. Removal of contaminating genomic DNA is recommended for gene expression studies where microarray or quantitative RT-PCR techniques are employed. Using our Absolutely RNAŽ kits gives you the flexibility in primer design since you don’t have to worry about genomic DNA contamination.

Absolutely RNAŽ Miniprep Kit

• Flexible Starting Amounts Of Cells Or Tissue
• 10⁵ - 10⁷ cells
• 5 - 40 mg of tissue
• 50 ul elution volume
• Includes DNase

The Absolutely RNAŽ Miniprep Kit is the only kit that produces high yields of total RNA for RT-PCR, QRT-PCR, microarray target samples, Northern blot analysis, and in vitro transcription reactions. Most kits on the market have a very narrow specification for tissue input amount. Samples that lie outside this defined range often generate poor or even no yield of RNA. In contrast, our Absolutely RNA Miniprep kit method accommodates a wide range of tissue and cell amounts without compromising RNA yield or integrity. Additionally, our kit is ideal for purification of in vitro transcription reactions. Coupled with an effortless on-column DNA removal step only our kit allows you to purify, DNA-free RNA from cells or tissues.

Absolutely RNAŽ Microprep Kit

• Optimized For Cultured Cells
• Up to 5 x 10⁵ cells
• 30 ul elution volume
• Laser Microdissection Samples
• Includes DNase

The Absolutely RNAŽ Microprep Kit isolates total RNA from small starting samples and LMD samples. This kit is designed for samples containing up to 5 x 10⁵ cells, making the isolated RNA ideal for quantitative RT-PCR and microarray target generation.

Absolutely RNAŽ 96 Microprep Kit

• High Throughput Total RNA Purification
• 10 cells to 5 x 10⁵ cells
• 30 ul elution volume
• Compatible with common 96-well plate equiment, such as the BiomekŽ 2000 Robot
• Includes DNase

The Absolutely RNAŽ 96 Microprep Kit efficiently isolates high-quality total RNA from a variety of cultured cells from 10 cells up to 5 x 10⁵ cells. This kit is perfect for RNA purification for all of your high-throughput purification needs. The purified RNA can be used for RT-PCR, QRT-PCR, and expression analysis studies.

Absolutely RNAŽ Nanoprep Kit

• RNA Isolation from a Single Cell
• 1 x 10⁴ cells down to a single cell
• 10 ul elution volume
• Extremely Precious and Small Populations of Laser Microdissected Cells
• Includes DNase

The Absolutely RNAŽ Nanoprep Kit is the only kit qualified to isolate RNA from a single cell. This kit yield high-quality, concentrationed total RNA in a small 10-ul elution volume. Moreover, this kit is specially designed to isolate total RNA from the smallest amounts of starting material down to a single cell.

Absolutely RNAŽ FFPE kit

• Reliable RNA Purification from FFPE Tissues
• High yields of unmodified and QRT-PCR amplifiable RNA
• Kit offers you the ability to cross compare samples and experiments
• Includes Dnase

Our Absolutely RNAŽ FFPE Kit offers reliable RNA purification from paraffin-embedded samples, with an easy-to-use, optimized, and non-toxic method. Additionally, we have optimized our kit method to remove the chemical modifications to the RNA molecules so that the high yields of unmodified RNA are obtained. Because there is a high degree of variability in working with FFPE tissues, we also include our StratageneŽ QPCR Human Universal Reference RNA standard to control for this variability so you can produce meaningful quantitative reverse-transcriptase PCR (QRT-PCR) data. We include all of the necessary reagents from de-paraffinization to elution of pure RNA so there are no extra purchases required.